Different triggers spark stroke, injuries, and neurodegenerative diseases, but the molecular chain of events responsible for brain cell death in these conditions are the same. Johns Hopkins researchers have isolated the single protein at the end of the chain that delivers the fatal blow and hacks up a cell's DNA.
tac dung thuoc chua viem dai trang
Nucleus of a cell undergoing parthanatos cell death.
Image credit: Yingfei Wang and I-Hsun Wu, Johns Hopkins Medicine
The findings - published in the journal Science - could pave the way for new therapies to stop the process in its tracks and potentially prevent brain cell death.
thuoc chua viem dai trang mua o dau
Research partners Dr. Ted Dawson, Ph.D., now director of the Institute for Cell Engineering at the Johns Hopkins University School of Medicine, and Valina Dawson, Ph.D., professor of neurology, and their research group conducted experiments on laboratory-grown cells to determine the culprit in the event chain that ultimately causes cell death.
The new research builds on a growing body of knowledge of a distinct form of programmed brain cell death dubbed "parthanatos" by the team in earlier work to distinguish it from other types of cell death, such as apoptosis, necrosis, or autophagic death.
The research teams found that while stroke, injury, Alzheimer's disease, Parkinson's disease, and Huntington's disease have very different causes and symptoms, they share the brain cell death mechanism, parthanatos, and PARP, an enzyme involved in the process.
"I can't overemphasize what an important form of cell death it is; it plays a role in almost all forms of cellular injury," says Dr. Dawson. The combined research groups have spent years breaking down each link in the parthanatos chain of events and tracing what roles proteins play in the process.
Previous research indicated that when a protein - mitochondrial apoptosis-inducing factor (AIF) - moves from its residing location in the energy-producing mitochondria of the cell to the nucleus, it causes the genome housed in the nucleus to be carved up, leading to cell death.
bifina
MIF protein 'chops up DNA,' triggers parthanatos cell deathWhile the transfer of AIF into the nucleus leads to cell death, AIF is not responsible for the DNA being carved. Yingfei Wang, Ph.D., an assistant professor at the University of Texas Southwestern Medical Center, screened thousands of human proteins to identify those that strongly interacted with AIF and could, therefore, be responsible for cutting up the DNA.
Wang identified 160 possible protein candidates and stopped each of them being produced one by one in lab-grown human cells, in order to determine whether cell death would be prevented if one protein was eliminated.
Of all the 160 proteins, the team identified macrophage migration inhibitory factor (MIF) at the heart of the cell-death process
tac dung thuoc chua viem dai trang
Nucleus of a cell undergoing parthanatos cell death.
Image credit: Yingfei Wang and I-Hsun Wu, Johns Hopkins Medicine
The findings - published in the journal Science - could pave the way for new therapies to stop the process in its tracks and potentially prevent brain cell death.
thuoc chua viem dai trang mua o dau
Research partners Dr. Ted Dawson, Ph.D., now director of the Institute for Cell Engineering at the Johns Hopkins University School of Medicine, and Valina Dawson, Ph.D., professor of neurology, and their research group conducted experiments on laboratory-grown cells to determine the culprit in the event chain that ultimately causes cell death.
The new research builds on a growing body of knowledge of a distinct form of programmed brain cell death dubbed "parthanatos" by the team in earlier work to distinguish it from other types of cell death, such as apoptosis, necrosis, or autophagic death.
The research teams found that while stroke, injury, Alzheimer's disease, Parkinson's disease, and Huntington's disease have very different causes and symptoms, they share the brain cell death mechanism, parthanatos, and PARP, an enzyme involved in the process.
"I can't overemphasize what an important form of cell death it is; it plays a role in almost all forms of cellular injury," says Dr. Dawson. The combined research groups have spent years breaking down each link in the parthanatos chain of events and tracing what roles proteins play in the process.
Previous research indicated that when a protein - mitochondrial apoptosis-inducing factor (AIF) - moves from its residing location in the energy-producing mitochondria of the cell to the nucleus, it causes the genome housed in the nucleus to be carved up, leading to cell death.
bifina
MIF protein 'chops up DNA,' triggers parthanatos cell deathWhile the transfer of AIF into the nucleus leads to cell death, AIF is not responsible for the DNA being carved. Yingfei Wang, Ph.D., an assistant professor at the University of Texas Southwestern Medical Center, screened thousands of human proteins to identify those that strongly interacted with AIF and could, therefore, be responsible for cutting up the DNA.
Wang identified 160 possible protein candidates and stopped each of them being produced one by one in lab-grown human cells, in order to determine whether cell death would be prevented if one protein was eliminated.
Of all the 160 proteins, the team identified macrophage migration inhibitory factor (MIF) at the heart of the cell-death process